Part:BBa_K416000:Experience

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Please enter how you used this part and how it worked out.

Applications of BBa_K416000

The 2011 UCSF iGEM team used the Aga2 display protein to create cell-cell adhesion. Two adhesive molecules (usually capable of creating homotypic interactions) were displayed on the cell surface by creating an Aga2-adhesive molecule fusion protein.

The proteins we used were:
Hwp1, a C. albicans fungal adhesion known to participate in biofilm formation
and
E-cadherin, a mammalian cell adhesion that creates stable multicellular interactions

These parts were submitted to the registry and information on the adhesion seen using these protein fusions can be seen on their parts pages.

BBa_K644000 Extracellular Domain of E-Cadherin (Mouse) BBa_K644001 Candida albicans hyphal wall protein 1 An improved version of this part is BBa_K2663005

User Reviews

UNIQ423a4fbf5b2f4d33-partinfo-00000000-QINU

No review score entered. RussellDurrett

The Aga2 biobrick has been shown to function similarly to that used in yeast surface display. The previous picture shows concurrent imaging of an Aga2:GFP fusion and a yeast membrane dye. As you can see in the merge photo the Aga2 biobrick successfully localizes GFP to the cellular surface.

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UNIQ423a4fbf5b2f4d33-partinfo-00000002-QINU